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protein chip array  (RayBiotech inc)


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    Structured Review

    RayBiotech inc protein chip array
    Protein Chip Array, supplied by RayBiotech inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protein+chip+array/Human+Chemokine+Array+GS1/pmc13378945-39-30-32
    Average 94 stars, based on 1 article reviews
    protein chip array - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Expressing:

    Article Title: Neuronal DJ-1 regulates microglial activation in Parkinson’s disease
    Article Snippet: .. The expression profiles of 15 chemokine-related genes were analyzed using a mouse custom polymerase chain reaction (PCR) array kit (Biotnt, Hangzhou, Zhejiang, China), while chemokine levels were assessed with a protein-chip array (Raybiotech, Norcross, GA, USA), each in accordance with the respective manufacturer’s instructions. .. The protein array results were scanned using an ImageQuant LAS4000 Scanner (Cytiva, Marlborough, MA, USA).

    Polymerase Chain Reaction:

    Article Title: Neuronal DJ-1 regulates microglial activation in Parkinson’s disease
    Article Snippet: .. The expression profiles of 15 chemokine-related genes were analyzed using a mouse custom polymerase chain reaction (PCR) array kit (Biotnt, Hangzhou, Zhejiang, China), while chemokine levels were assessed with a protein-chip array (Raybiotech, Norcross, GA, USA), each in accordance with the respective manufacturer’s instructions. .. The protein array results were scanned using an ImageQuant LAS4000 Scanner (Cytiva, Marlborough, MA, USA).



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    Bio-Rad seldi protein chip arrays
    Identification of serum proteins by immunodepletion and western blotting. Proteins representing peaks at m/z 6624, m/z 8916 and m/z 13870 were immunodepleted from serum extracts using monoclonal antibodies against (A) apolipoprotein CI (ApoCI), (B) C3a/ C3a des-arginine anaphylatoxin (C3a-desArg) or (C) transthyretin. The starting material (Starting, upper panel), the immunodepleted sample (Depleted, middle panel) and the eluted fraction (Recovered, lower panel) were analyzed on NP20 arrays by surface-enhanced laser desorption/ionization <t>time-of-flight</t> <t>(SELDI-TOF)</t> mass spectrometry (MS). (D) Western blotting for ApoCI, C3a/C3a-desArg and transthyretin were performed on four healthy volunteer (HV) samples and four breast cancer patient (BC) samples. (E) Mean band densities (+standard deviation (SD)) derived from the blots shown in panel D (n = 4 for all groups). (F-H) Association between SELDI peak intensities and western blotting band densities for individual HV and BC serum samples (n = 8), indicating strong correlations for (F) ApoCI, (G) C3a-desArg and (H) transthyretin.
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    Image Search Results


    Identification of serum proteins by immunodepletion and western blotting. Proteins representing peaks at m/z 6624, m/z 8916 and m/z 13870 were immunodepleted from serum extracts using monoclonal antibodies against (A) apolipoprotein CI (ApoCI), (B) C3a/ C3a des-arginine anaphylatoxin (C3a-desArg) or (C) transthyretin. The starting material (Starting, upper panel), the immunodepleted sample (Depleted, middle panel) and the eluted fraction (Recovered, lower panel) were analyzed on NP20 arrays by surface-enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectrometry (MS). (D) Western blotting for ApoCI, C3a/C3a-desArg and transthyretin were performed on four healthy volunteer (HV) samples and four breast cancer patient (BC) samples. (E) Mean band densities (+standard deviation (SD)) derived from the blots shown in panel D (n = 4 for all groups). (F-H) Association between SELDI peak intensities and western blotting band densities for individual HV and BC serum samples (n = 8), indicating strong correlations for (F) ApoCI, (G) C3a-desArg and (H) transthyretin.

    Journal: Breast Cancer Research : BCR

    Article Title: Novel serum protein biomarker panel revealed by mass spectrometry and its prognostic value in breast cancer

    doi: 10.1186/bcr3676

    Figure Lengend Snippet: Identification of serum proteins by immunodepletion and western blotting. Proteins representing peaks at m/z 6624, m/z 8916 and m/z 13870 were immunodepleted from serum extracts using monoclonal antibodies against (A) apolipoprotein CI (ApoCI), (B) C3a/ C3a des-arginine anaphylatoxin (C3a-desArg) or (C) transthyretin. The starting material (Starting, upper panel), the immunodepleted sample (Depleted, middle panel) and the eluted fraction (Recovered, lower panel) were analyzed on NP20 arrays by surface-enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectrometry (MS). (D) Western blotting for ApoCI, C3a/C3a-desArg and transthyretin were performed on four healthy volunteer (HV) samples and four breast cancer patient (BC) samples. (E) Mean band densities (+standard deviation (SD)) derived from the blots shown in panel D (n = 4 for all groups). (F-H) Association between SELDI peak intensities and western blotting band densities for individual HV and BC serum samples (n = 8), indicating strong correlations for (F) ApoCI, (G) C3a-desArg and (H) transthyretin.

    Article Snippet: All serum samples were initially denatured in buffer containing 8 M urea, 1% CHAPS (3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfate) and analyzed by TOF MS on SELDI protein chip arrays (Bio-Rad, Hercules, CA, USA) as previously described [ ].

    Techniques: Western Blot, Mass Spectrometry, Standard Deviation, Derivative Assay